Antimicrobial Efficacies of Brassica Napus L. Essential Oils Nanoparticles Composites

Polylactide based essential oil films were formulated by incorporating polyethylene glycol, nanopowder (zinc oxide), and essential oil by solvent casting method. The films were tested against pathogens for their antibacterial activity. The effectiveness of selected oil-nanomaterial based film was tested by performing the tests. In vitro antibacterial efficacies of nanopowders/essential oil were determined by the decimal reduction concentrations and the minimum bactericidal concentrations for the pathogens. In a typical process, Brassica napus extract was obtained from supercritical fluid extraction using pressurized carbon dioxide as solvent. The composition of the essential oil was analyzed by gas chromatography (GC) and gas chromatography-mass spectrophotometry (GC-MS). 39 compounds were identified in the oil. The major compounds of the oil were 1,3,6,10-Cyclotetradecatetraene, 3,7,11-trimethyl-14- (1-methylethyl) - 30,07%, Cyclohexanone, 5 -methyl-2- (1-methylethylidene) - 12.91%, 3,4-Methylenedioxypropiophenone - 9,67%, Hexadecanoic acid, ethyl ester - 8.28%, Octacosanol - 5,50%, 11,15-Tetramethylhexadeca-1,3,6,10,14-pentaene - 4,55% and 1,6,10,14-Hexadecatetraen-3-ol, 3,7,11,15-tetramethyl – 3,14 %.