Antibacterial and Antibiofilm Activity of Bacteria Mediated Synthesized Fe3O4 nanoparticles Using Bacillus Coagulans

There are several types of nanoparticles, and it may be that metals and their oxides have been important since ancient times for medical uses, Iron Oxide NPs was preferred among all for its unique properties, supernatant of gram positive Bacillus Coagulans bacteria was employed in synthesis of IONPs as stabilizing and bio reducing agent, the synthesized iron oxide NPs were characterized by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD),Scanning electron microscope (SEM) and Atomic force microscope(AFM) ,the short incubation time and rapid precipitated in the synthesis protocol of NPs resulting in fine agglomeration and small size of particles were calculated from (XRD) in avenge about 15.2 nm , and to reveal the activity of IONPs as an antibacterial and antibiofilm formation agent respectively, it was experimented against Uropathogenic E.coli bacteria isolated from urinary tract infection (UTIs) patients, by well diffusion method and it was exhibited potent antibacterial activity in depends on concentration ,also Fe3O4 NPs was displayed strong antibiofilm activity were test in concentration of MIC at 150 μg/ml through two methods, congo red and tube method .