Title of article :
Ginger Extract Modulates the Production of Chemokines CCL17, CCL20, CCL22, and CXCL10 and the Gene Expression of Their Receptors in Peripheral Blood Mononuclear Cells from Peptic Ulcer Patients Infected with Helicobacter pylori
Author/Authors :
Jalalpour ، Shila Molecular Medicine Research Center, Research Institute of Basic Medical Sciences - Rafsanjan University of Medical Sciences , Mirzaee ، Vahid Department of Internal Medicine - Rafsanjan University of Medical Sciences , Taheri ، Mohammad Department of Microbiology - School of Medicine - Hamadan University of Medical Sciences , Sheikh Fathollahi ، Mahmood Rajaie Cardiovascular Medical and Research Center - Iran University of Medical Sciences , Khorramdelazad ، Hossein Molecular Medicine Research Center, Research Institute of Basic Medical Sciences - Rafsanjan University of Medical Sciences , Jafarzadeh ، Abdollah Molecular Medicine Research Center, Research Institute of Basic Medical Sciences - Rafsanjan University of Medical Sciences
Abstract :
Background: The imbalanced expression of chemokines plays critical role in the development of Helicobacter pylori-mediated complications. Objectives: Our aim was to determine ginger extract (GE) effects on the expression of chemokines CCL17, CCL20, CCL22, and CXCL10, as well as CCR4, CCR6, and CXCR3 receptors by peripheral blood mononuclear cells (PBMCs) from H. pylori -infected patients with peptic ulcer (PU). Methods: Peripheral blood mononuclear cells were obtained from 20 patients with H. pylori-associated PU, 20 H. pylori-infected asymptomatic subjects (HAS), and 20 non-infected healthy subjects (NHS). The PBMCs were stimulated by 10 µg/mL of H. pylori-derived crude extract (HPCE) in the presence of 0, 10, 20, and 30 µg/mL of GE. After 36 hours, the supernatant and the RNA extracted from the cells were tested for chemokine concentration and chemokine receptor expression using ELISA and real-time PCR techniques, respectively. Results: In PU patients, treating HPCE-stimulated PBMCs with 10, 20, or 30 µg/mL GE reduced the production of CXCL10 (1.47, 1.5, and 1.53 folds, respectively, P 0.001 for all), CCL20 (1.44, 1.62, and 1.65 folds, respectively, P 0.003), and treatment with 30 µg/mL GE increased CCL17 (1.28-fold, P 0.001) and CCL22 (1.59-fold, P 0.001) production compared with untreated HPCE-stimulated PBMCs. In PU patients, the HPCE-stimulated PBMCs treated with 10, 20, or 30 µg/mL GE expressed lower levels of CXCR3 (1.9, 3, and 3.5 folds, respectively, P 0.001) and CCR6 (2.3, 2.7, and 2.8 folds, respectively, P 0.002) while treating with 10 µg/mL GE upregulated CCR4 (1.7 fold, P = 0.003) compared with untreated HPCE-stimulated PBMCs. Conclusions: Ginger extract modulated the expression of chemokines and their receptors in the PBMCs derived from H. pylori-infected PU patients. The therapeutic potentials of ginger for treating HP-related complications need to be further explored
Keywords :
Helicobacter pylori , Peptic Ulcer , Ginger , Chemokines , Chemokine Receptor , PBMCs
