Dissmination of TEM and SHV Extended-Spectrum Beta-Lactamases in Clinical Isolates of Escherichia coli

Purpose: To examine the dissemination of blaTEM and blaSHV genes in Escherichiacoli isolate from patients with significant bacteriuria.Methods: During the study period, antibiotic susceptibility patterns were studied in atotal of 38 isolates of E. coli. The isolates were also examined to determining thepresence of blaTEM and blaSHV genes by PCR assay. Conjugation experiments wereperformed by using rifampicin resistance E. coli MM294 as the recipient. Results: All isolates were resistance to at least five of the antibiotics tested (multidrug resistance, MDR) and sensitive to imipenem. PCR assay revealed that 57.1% and14.3% of the isolates carried blaTEM and blaSHV genes, respectively, additionally 9.5% of the isolates harbored both genes. Conjugation experiments and PCR trials confirmed that the blaTEM positive E. coli isolates tested were able to transfer this gene in transconjugants. Conclusions: There was a high occurrence of MDR isolates in patients with significant bacteriuria and the blaTEM gene was common among isolates