The using of antisperm antibody assay as a predictive diagnostic test of male infertility

Assessment of male infertility is the first step in resolving this worldwide problem . The aim of the present study is to investigate the predictive value of using ELISA (Enzyme Linked Immuno-Sorbent Assay) test for serum and seminal plasma Antisperm antibodies (ASA) as a complementary tool to assist in diagnosis of infertility . This study was carried out between January 2013 and November 2013 including one hundred (100) selected infertile men who attended to fertility Center in Al-Sadr Medical City . The study also included twenty (20) healthy volunteer fertile as a control . The results showed that the incidence of serum and seminal ASA in infertile men is significantly (p 0.05) higher than that in control fertile men. the incidence of serum and seminal ASA is significantly (p 0.05) higher in normozoospermic patients than that in control fertile men . There was a high significant negative correlation (p 0.01) between the concentration of serum and seminal ASA in infertile men and each of : sperm motility, progressive motility, while a high significant positive correlation (p 0.01) is seen between serum and seminal ASA in infertile men and sperm agglutination, and a significant positive correlation (p 0.05) with seminal WBC count . The study concluded that ELISA ASA assay can be routinely used as a complementary test to diagnose infertility.