Title of article :
CircRNA-011235 Counteracts The Deleterious Effect of Irradiation Treatment on Bone Mesenchymal Stem Cells by Regulating The miR-741-3p/CDK6 Pathway
Author/Authors :
Wen ، Xianhui Department of Blood Transfusion - Third Xiangya Hospital of Central South University , Xie ، Hebin Research and Teaching Department - Changsha Central Hospital , Gui ، Rong Department of Blood Transfusion - Third Xiangya Hospital of Central South University , Nie ، Xinmin Third Xiangya Hospital of Central South University , Shan ، Dongyong Hunan Cancer Hospital and The Affiliated Cancer Hospital of Xiangya School of Medicine - Central South University , Huang ، Rong Department of Blood Transfusion - Third Xiangya Hospital of Central South University , Deng ، Hongyu Hunan Cancer Hospital and The Affiliated Cancer Hospital of Xiangya School of Medicine - Central South University , Zhang ، Junhua Department of Blood Transfusion - Third Xiangya Hospital of Central South University
Abstract :
Objective: The present work was aimed at uncovering the effect of circRNA-011235 (circ-011235) on irradiation-induced bone mesenchymal stem cells (BMSCs) injury and its regulatory mechanism, with a view to establish a scientific basis for its possible medical applications. Materials and Methods: In this experimental study, after irradiation with different doses (0, 2, 4, 6 GY), the relative expression levels of circ-011235, miR-741-3p, and cyclin-dependent kinases 6 (CDK6) were detected in the BMSCs, using the real time-quantitative polymerase chain reaction (RT-qPCR). The overexpression effects of circ-011235 and CDK6 on the cell proliferation in irradiation-treated BMSCs were measured by the Cell Counting Kit-8 (CCK8) assay. And also, their effects on the cell cycle were evaluated by flow cytometry. RT-qPCR and immunoblotting were performed to detect the effects of pcDNA-circ-011235 and pcDNA-CDK6 on the expression of cyclin D1 and cyclindependent kinases 4 (CDK4) at the gene and protein levels, respectively. Results: Irradiation treatment elevated the expression of circ-011235 and CDK6, but reduced miR-741-3p expression in the BMSCs with a dose-dependent effect. The proliferation of BMSCs was significantly inhibited in the irradiation treatment group, while the overexpression of circ-011235 and CDK6 effectively attenuated this inhibition. Also, overexpression of circ-011235 and CDK6 elevated the expression of cyclin D1 in irradiation-treated BMSCs, but had no significant effect on the CDK4 expression. Conclusion: Our results demonstrated that circ-011235 up-regulated the expression of cyclin D1 via miR-741-3p/ CDK6 signal pathway, thereby promoting cell cycle progression and proliferation of irradiation-treated BMSCs. This finding suggested circ-011235/ miR-741-3p/CDK6 pathway exerted a protective role in the response to irradiation and will be a potential new target for future research on the mechanism involved in the resistance of BMSCs to radiation.
Keywords :
Bone Mesenchymal Stem Cell , CDK6 , Cell Cycle , Irradiation ,
Journal title :
Cell Journal (Yakhteh)
Journal title :
Cell Journal (Yakhteh)
