Author/Authors :
Jalili ، Arsalan Department of Stem Cells and Developmental Biology, Department of Applied Cell Sciences - Cell Science Research Center, Faculty of Basic Sciences and Advanced Medical Technologies - Royan Institute for Stem Cell Biology and Technology , Hajifathali ، Abbas Hematopoeitic Stem Cell Research Center - Shahid Beheshti University of Medical Sciences , Bereimipour ، Ahmad Department of Stem Cells and Developmental Biology - Cell Science Research Center - Royan Institute for Stem Cell Biology and Technology , Roshandel ، Elham Hematopoeitic Stem Cell Research Center - Shahid Beheshti University of Medical Sciences , Aghdami ، Nasser Department of Regenerative Medicine - Cell Science Research Center - Royan Institute for Stem Cell Biology and Technology
Abstract :
Objective: Different Cell Culture medias can affect the expansion of T cells. The aim of this study is to assess signaling pathways, protein interactions and genes in T cells cultured in different common T cell expansion medias to select the best candidate. Materials and Methods: In this in silico observational study, with the use of bioinformatics analysis and the use of enrichment databases, gene expression profiles were investigated using microarray analysis. Results: The results of this study were the joint selection of 26 upregulated genes and 59 downregulated genes that were involved in SREBP control of lipid synthesis, co-stimulatory signal during T-cell activation mitosis and chromosome dynamics, telomeres, telomerase, and cellular aging signal pathways. Conclusion: Using bioinformatics analyzes, integrated and regular genes were selected as common genes CD80, LST1, ATM and ITM2B 4-1BBL, Akt inhibitor, interleukin 7 and 15 expansion media.
