Title of article
Effects of trehalose on NFE2L2, catalase, and superoxide dismutase in the kidney of aged rats
Author/Authors
Hozhabri ، Yaser Neuroscience Research Center, Institute of Neuropharmacology - Kerman University of Medical Sciences , Sadeghi ، Asie Department of Clinical Biochemistry - Faculty of Medicine - Kerman University of Medical Sciences , Nazari-Robati ، Mahdieh Department of Clinical Biochemistry - Neuroscience Research Center, Institute of Neuropharmacology, Faculty of Medicine - Kerman University of Medical Sciences , Bahri ، Faegheh Department of Clinical Biochemistry - Faculty of Medicine - Kerman University of Medical Sciences , Salimi ، Fouzieh Department of Clinical Biochemistry - Faculty of Medicine - Kerman University of Medical Sciences , Abolhassani ، Moslem Department of Clinical Biochemistry - Faculty of Medicine - Kerman University of Medical Sciences , Mohammadi ، Abbas Department of Clinical Biochemistry - Faculty of Medicine - Kerman University of Medical Sciences
From page
29
To page
36
Abstract
Aging is associated with an increase in oxidative stress, which damages organs such as the kidney. Trehalose has abundant beneficial activities including antioxidative effects. This study aimed to investigate the effects of trehalose on several antioxidant parameters of the aged kidney .Wistar rats were divided into three groups: young (4 months), aged (24 months), and aged-trehalose. The third group was treated with 2% trehalose for one month. The expression of target genes and enzyme activities in the kidney of the animals were evaluated by quantitative polymerase chain reaction (qPCR) and enzyme colorimetric procedures, respectively. Protein levels of NFE2L2 showed a 50% reduction in aged rats compared to young rats (P 0.001), which was restored by trehalose intervention. In addition, the activity and mRNA levels of catalase (CAT) increased in aged rats while treatment with trehalose reversed this trend. On the other hand, superoxide dismutase (SOD) activity was reduced in the kidneys of aged rats but was not affected by trehalose intervention . It is concluded that trehalose supplementation alleviates the antioxidant system impairments in the kidneys of aged rats. However, further investigations are needed to thoroughly describe the antioxidative impacts of trehalose on the kidneys during aging.
Keywords
Trehalose , Oxidative stress , NFE2L2 , SOD , CAT
Journal title
Molecular Biology Research Communications
Journal title
Molecular Biology Research Communications
Record number
2708965
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