Abstract :
A simple and effective technique for detecting catechol by the generation of white precipitate utilizing the reaction of potassium dichromate with catechol in sulfuric acid medium, which is characterized by its speed and sensitivity. The NAG-4SX3-3D analyzer was utilized to measure the incident light attenuation impacting on the precipitate surface grains to quantify turbidity (0-180 degree), the snow led [LED] (blue band 400-480 nm, green band 443-600 nm, and red band 660-697 nm) was utilized to irradiate precipitate particles throughout the processes to get a transducer energy response in mV vs. time. The appropriate parameter was researched in order to increase the sensitivity of the newly devised technique. For catechol measurement, the linear range (0.01-27) millimol.L-1 with (r=0.9996), (correlation coefficient), percentage linearity (R2 percent=99.93), and RSD % for the repetition (n=6) were significantly lower than 0.2 percent (0.7, 15 millimol .L-1), with L.O.D. = 154.14 ng/sample from the progressive dilution across the calibration graph’s lowest concentration linear dynamic range. The suggested strategy was compared to the traditional method (UV-spectrophotometric at λmax=275 nm and turbidimetric method). It may be concluded that in addition to the technique’s sensitivity (developed) and the employment of few chemicals, the approach is also characterized by a dynamic system, which prevents precipitated particle setting during measurements as compared to the conventional reference method’s 10 mm irradiation. In addition, continuous dilution in CFIA allows for dealing with high or low concentrations, allowing for a wider range of applications. Based on the foregoing, the developed technique is deemed to be the most appropriate for catechol molecules when compared to the reference methods.
