Title of article :
Spectrophotometric determination of phenobarbital in pharmaceutical preparation using gold nanoparticles
Author/Authors :
Al Samarrai ، Eman Thiab Department of Chemistry - College of Education - University of Samarra , Alwan ، Liqaa H. Department of Chemistry - College of Education - University of Samarra , Al-Haddad ، Suha Abdullah Hussein Department of Chemistry - College of Education - University of Samarra , Al Samarrai ، Mustafa Hamed Department of Chemistry - College of Education - University of Samarra , Al-Obaidi ، Muhannad Salim Miteb Department of Chemistry - College of Education - University of Samarra , Al Samarrai ، Othman Rashid Department of Chemistry - College of Education - University of Samarra
Abstract :
A simple, rapid and accurate spectrophotometric method is proposed to determine Phenobarbital (PHB) based on the coupling of 2,6-Dichloroindophenol sodium salt hydrate (DSH) with PHB to give a new ligand which reacts with gold nanoparticles in alkaline medium at (30-40°C) to give an intense blue-green colored chelate complex having maximum absorption at 600nm. The optimization of the experimental conditions is described. Beer’s Law is obeyed at concentration range up to 5-45μg/ml with molar absorptivity 2368.797 L/mol.cm, Sandell s index was 0.09803 (μg/cm²) The correlation coefficient, limit of detection and limit of quantification were 0.9984, 1.3002μg/ml and 4.3342μg/ml, respectively. The method has been successfully used to determine the Phenobarbital concentration in pharmaceutical preparation.
Keywords :
Phenobarbital , 2,6 , dichloroindophenol sodium salt hydrate , gold nanoparticles , spectrophotometric method
Journal title :
Eurasian Chemical Communications
Journal title :
Eurasian Chemical Communications
