Title of article :
Measuring of free endotoxin in alum-precipitated vaccine of haemorrhagic septicaemia by limulus amebocyte lysate test
Author/Authors :
Jabbari, AR Razi Vaccine and Serum Research Institute, Karadj - Department of Aerobic Veterinary Bacterial Vaccine, ايران , Moazeni Jula, GR Razi Vaccine and Serum Research Institute, Karadj - Department of Aerobic Veterinary Bacterial Vaccine, ايران
Abstract :
Haemorrhagic septicaemia (HS) vaccine which is prepared in Razi Institute is used in endemic areas of Iran. Aluminum-hydroxide gel was used as adjuvant for preparing this vaccine. Post-vaccinal shock reactions were the main complaint after use of this vaccine. In a previous study, we could improve the vaccine by alum-precipitation Pasteurella multocida cells and removing the liquid phase. In this study, the amount of free endotoxin in aluminum-hydroxide and alum-HS vaccines was determined. It was found that endotoxin level was considerably decreased from 0.22 EU/ml to 0.03 EU/ml after alum-precipitation
Keywords :
Haemorrhagic septicaemia vaccine , Endotoxin , Shock reaction , LAL test
Journal title :
Iranian Journal of Veterinary Research (IJVR)
Journal title :
Iranian Journal of Veterinary Research (IJVR)
