Effect of Diode Laser (805) nm on alpha-toxin production and antibiotic sensitivity of Staphylococcus aureus

The effect of low level laser radiation on Staphylococcus aureus with different exposure times has been studied. Thirty skin samples [swabs] were collected from patients with burn and wound infections of Al-yarmouk teaching hospital, during the period from November 2010 to March 2011. Ten isolates of S. aureus were identified by their cultural characteristics, microscopic examination, and biochemical tests. The ten isolates were exposed to diode laser [805nm] at different exposure times [1min., 3min. and 5min.]. The activity of bacteria to produce alpha toxin and its sensitivity to antibiotics were determined before and after irradiation. The result of alpha toxin production of ten irradiated isolates were illustrated that toxin production was decreases after different time of exposure [1, 3 and 5 min.] of irradiation. The effect of diode laser on the sensitivity of S. aureus to antibiotics discs shows slightly increase in the diameter of inhibition zone to these antibiotics at different time of exposure.