Title of article :
Identification of Agrobacterium tumefaciens Strains by PCR-RFLP Analysis of the 16S-rDNA
Author/Authors :
Al-Karablieh, Nihayah University of Jordan - Faculty of Agriculture - Department of Plant Protection, Jordan , Khlaif, Hamed University of Jordan - Faculty of Agriculture - Department of Plant Protection, Jordan , Al-Banna, Lama University of Jordan - Faculty of Agriculture - Department of Plant Protection, Jordan
Abstract :
16S-rDNA analysis of 30 Agrobacterium tumefaciens Jordanian isolates belonging to 1, 2, 3 and intermediate biotypes, were characterized by the restriction fragment length polymorphism analysis of PCR product. Two reference cultures of A. tumefaciens were used for comparison. The profile obtained by the analysis formed three main clusters confirmed the biotyping of the strains, and allocated intermediate biotype with biotype 1 strains as a typical biotype 1strains, also, higher genetic distances were found within biotype 1 strains
Keywords :
Agrobacterium , 16S , rDNA , PCR , RFLP , Dendrogram , Electrophoresis
Journal title :
Jordan Journal of Agricultural Sciences
Journal title :
Jordan Journal of Agricultural Sciences
