Author/Authors :
Tabet-Aoul, N. Universite d Oran - Faculte des Sciences - Departement de Biotechnologie, Algeria , Ait-Yahia, R. Universite des Sciences et de la Technologie - Faculte des Sciences - Departernent de Biologie Appliquee, Algeria , Derrar, A. Universite des Sciences et de la Technologie - Faculte des Sciences - Departernent de Biologie Appliquee, Algeria , Boushaba, N. Universite des Sciences et de la Technologie - Faculte des Sciences - Departernent de Biologie Appliquee, Algeria , Tabet-Aoul, K. Laboratoire de Genetique des Poissons, INRA, France , Saidi-Mehtar Universite d Oran - Faculte des Sciences - Departement de Biotechnologie, Algeria
Abstract :
A panel of 24 hamster-sheep somatic cell hybrids was investigated for sheep gene mapping to localize new
microsatellite markers. In order to achieve this goal, sixty microsatellites were selected based on data available
from different livestock maps of sheep, cattle and goat. Synteny was identified by statistical rules. Results
showed 18 new microsatellite localizations on eight (8) sheep chromosomes. Some microsatellites previously
localized on sheep chromosomes were used towards a better characterization of the panel. Furthermore, this
study provided indications about the existence of new breakpoints in ovine chromosomes present in the hybrid
lines.
Keywords :
genetic map , characterization , microsatellites , sheep , hamster-sheep somatic hybrids
