Title of article :
Impairment of sperm efficiency in mice following short-term nano-titanium dioxide exposure: An experimental study
Author/Authors :
Danafar ، Amirhossein Dr. Seyedhassani Medical Sciences Center , Khoradmehr ، Arezoo Persian Gulf Marine Biotechnology Research Center, Persian Gulf Biomedical Sciences Research Institute - Bushehr University of Medical Sciences , Hosseini Bondarabadi ، Mahya CNRS, LMGE - Université Clermont Auvergne , Mazaheri ، Fahime Research and Clinical Center of Infertility, Yazd Reproductive Science Institute, Medical Nanotechnology and Tissue Engineering Research Centre - Shahid Sadoughi University of Medical Sciences , Tamadon ، Amin Persian Gulf Marine Biotechnology Research Center, Persian Gulf Biomedical Sciences Research Institute - Bushehr University of Medical Sciences , Pourmasoumi ، Soheila Clinical Education and Research Unit, Clinical Research Development Unit (CRDU), - Moradi Hospital, Non-Communicable Diseases Research Center - Rafsanjan University of Medical Sciences , Gholizadeh ، Lida Research and Clinical Center of Infertility, Yazd Reproductive Science Institute - Shahid Sadoughi University of Medical Sciences , Moshrefi ، Mojgan Research and Clinical Center of Infertility, Yazd Reproductive Science Institute, Medical Nanotechnology and Tissue Engineering Research Centre - Shahid Sadoughi University of Medical Sciences , Halvaei ، Iman Department of Anatomical Sciences - Faculty of Medical Sciences - Tarbiat Modares University , Hosseini ، Akram Department of Anatomical Sciences - School of Medicine - Isfahan University of Medical Sciences , Golzadeh ، Jalal Stem Cell Biology Research Center, Yazd Reproductive Science Institute - Shahid Sadoughi University of Medical Sciences , Rahiminia ، Tahereh Gametogenesis Research Center, Fertility and Infertility Center - Kashan University of Medical Sciences , Anvari ، Morteza Department of Biology and Anatomical Sciences - Research and Clinical Center of Infertility, Yazd Reproductive Science Institute - Shahid Sadoughi University of Medical Sciences
Abstract :
Background: Titanium dioxide nanoparticles (TiO2NPs) are widely used in many compounds. Recent evidence has displayed some cytotoxic effects of TiO2NPs on male reproduction. Objective: The effects of TiO2NP administration on sperm parameters and chromatin and seminiferous histopathology of male mice were investigated. Materials and Methods: In this experimental study, 32 NMRI male mice (35 ± 3 gr, 8-12-weekold) were divided into four groups (n = 8/each): treated groups were fed orally with 2.5 (group I), 5 (group II) and 10 (group III) mg/kg/day TiO2NPs for 40 days and the control group received phosphate buffered saline. Sperm parameters, DNA integrity and chromatin quality were assessed using chromomycin A3, aniline blue, toluidine blue staining and TUNEL. Hematoxylin eosin staining was performed to measure spermatogenic cells and the total diameter of seminiferous tubules. Also, sex hormone and malondyaldehyde levels were measured. Results: Abnormal sperm tails rose in group III (28.87 ± 4.91) in comparison with the control group (12.75 ± 3.95). However, chromomycin A3 staining and TUNEL showed higher levels in group III in comparison with the control group, whereas aniline blue and toluidine blue staining showed no differences. A significantly lower spermatogenesis index and lumen parameters were observed in group III. Leydig cell numbers, cellular diameters and the area of the seminiferous tubules were lower in the treated groups. The testosterone level was also lower in these groups and the percentage of malondyaldehyde in the seminal fluid was higher. Conclusion: Exact mechanisms of TiO2NPs are not clear; however, cytotoxic and genotoxic effects of TiO2NPs may relate to oxidative stress. Given their widespread use, TiO2NPs should be a public health focus of attention.
Keywords :
Titanium dioxide , Spermatogenesis , Histology , Mouse , Chromatin , TUNEL
Journal title :
International Journal of Reproductive BioMedicine
Journal title :
International Journal of Reproductive BioMedicine
