Author/Authors :
Zakaria, Nur Husna Faculty of Medicine - Universiti Sultan Zainal Abidin (UniSZA), Terengganu, Malaysia , Mohamad, Nasir Faculty of Medicine - Universiti Sultan Zainal Abidin (UniSZA), Terengganu, Malaysia , Abu Bakar, Nor Hidayah Faculty of Medicine - Universiti Sultan Zainal Abidin (UniSZA), Terengganu, Malaysia , Hashim, Siti Norhajah Faculty of Medicine - Universiti Sultan Zainal Abidin (UniSZA), Terengganu, Malaysia , Shari, Halim Addiction Interest Group (AIG) - International Medical School - Management & Science University (MSU), Selangor, Malaysia , Mohd Adnan, Liyana Hazwani Faculty of Medicine - Universiti Sultan Zainal Abidin (UniSZA), Terengganu, Malaysia
Abstract :
Background: To determine the effects of Apis dorsata honey on the development of morphine tolerance and oxidative stress in rats.
Materials and Methods: A total of 40 male Sprague Dawley rats were injected (subcutaneous) with 10 mg/kg of morphine following oral administration of A. dorsata honey (0.5, 1.5, and 2.5g/kg). On day 15, the rats were euthanized, and the thalamus, spinal cord, and hippocampus were homogenized to assess iNOS and MDA using ELISA kits.
Results: The honey of A. dorsata significantly prevented morphine tolerance to analgesia in the hotplate test on Day 14 (p<0.05). The biochemical assessment showed that A. dorsata honey significantly reduced MDA formation in the brain regions compared to the morphine control group at dose 2.5g/kg. Elevation of iNOS caused by chronic morphine intake was reduced in A. dorsata honey co-treatment.
Conclusion: This study suggests the therapeutic role of A. dorsata honey in preventing morphine tolerance via inhibition of oxidative stress.
