Author/Authors :
Esmaeil Zadeh, Mohammad Reza Dept. of Pathobiology - Faculty of Public Health - Tehran University of Medical Sciences, Tehran , Sharifi Yazdi, Mohammad Kazem Zoonosis Research Center - Tehran University of Medical Sciences, Tehran , Rajabi, Zahra Food Microbiology Research Center - Tehran University of Medical Sciences, Tehran , Amin Harati, Farzaneh Dept. of Pathobiology - Faculty of Public Health - Tehran University of Medical Sciences, Tehran , Nikkhahi, Farhad Dept. of Pathobiology - Faculty of Public Health - Tehran University of Medical Sciences, Tehran , Sharifi Yazdi, Sara Faculty of Medicine - Tehran University of Medical Sciences, Tehran , Hassanpour, Gholamreza Center for Research of Endemic Parasites of Iran - Tehran University of Medical Sciences, Tehran , Monadi Sefidan, Alireza Dept. of Medical Laboratory Sciences - Faculty of Allied Medical Sciences - Tehran University of Medical Sciences, Tehran , Soltan Dallal, Mohammad Mehdi Dept. of Pathobiology - Faculty of Public Health - Tehran University of Medical Sciences, Tehran
Abstract :
Background & Objective: Phage therapy could be used as an alternative method to antibiotic treatments. The purpose of this study was to evaluate the antibacterial activities of isolated lytic bacteriophage against ciprofloxacin-resistant strain of Salmonella infanits in vitro conditions. Materials & Methods: The standard strain of Salmonella infantis and its specific bacteriophage was isolated by soft agar method. Phage susceptibility to heat and pH was evaluated by the Double-Layer Agar method. In vitro assay was carried out on human epithelial type 2 (HEp-2) cells to investigate the effect of bacteriophage on the cytotoxic and invasion of Salmonella infantis to human epithelial cells. Results: Head and tail morphology of bacteriophages against Salmonella infantis were identified by transmission electron microscopy and assigned to the Myoviridae family. The results of the double-layer agar assay showed that the titer of bacteriophages was 1.8×107 PFU/ml. bacteriophage was stable at 4 ֯C and the best quantification of bacteriophage was determined at pH=8. The isolated bacteriophage was specific for Salmonella infantis and had no lytic activity against other pathogenic bacteria. In the evaluation of the binding and invasion of Salmonella infantis to the HEp-2 cell line, as expected, the lytic activity of specific bacteriophage was observed following inoculation. Conclusion: Additional studies are needed for better understanding of the interaction between phage, microorganisms and human host before applying phage therapy on a large scale.
Keywords :
Salmonella , Salmonellosis , Lytic bacteriophage , Ciprofloxacin
