Author/Authors :
Amiri, Shahrokh Research Center of Psychiatry and Behavioral Sciences - Tabriz University of Medical Sciences - Tabriz, Iran , Farhang, Sara Research Center of Psychiatry and Behavioral Sciences - Tabriz University of Medical Sciences - Tabriz, Iran , Shekari Khaniani, Mahmoud Tabriz University of Medical Science - Tabriz, Iran , Mansouri Derakhshan, Sima Tabriz University of Medical Science - Tabriz, Iran , Zadfattah, Aziz Research Center of Psychiatry and Behavioral Sciences - Tabriz University of Medical Sciences - Tabriz, Iran , Mohammadi Bina, Zahra Research Center of Psychiatry and Behavioral Sciences - Tabriz University of Medical Sciences - Tabriz, Iran , Ghazipour, Fatemeh Research Center of Psychiatry and Behavioral Sciences - Tabriz University of Medical Sciences - Tabriz, Iran , Sardari, Narges Research Center of Psychiatry and Behavioral Sciences - Tabriz University of Medical Sciences - Tabriz, Iran , Barzegar, Habibeh Research Center of Psychiatry and Behavioral Sciences - Tabriz University of Medical Sciences - Tabriz, Iran , Mehdizadeh Fanid, Leila Department of Psychology - Faculty of Education and Psychology - University of Tabriz - Tabriz, Iran
Abstract :
Drug treatment is one of the most important treatments for attention deficit hyperactivity disorder (ADHD). The
DRD4 gene is a transporter and receptor coding gene of dopamine and is one of the most important genes under investigation in
the disorder and etiology of ADHD. In this study, the association between rs3758653 C/T andVNTRexon 3 repetition polymorphisms of
the DRD4 gene and the effects of methylphenidate were investigated in patients with ADHD disorder consuming methylphenidate.
Methods: The descriptive-analytical study was performed on 122 patients (5 - 18 years old) with ADHD who were treated with
methylphenidate. DNA was extracted using salting out method. Subsequently, the rs3758653 polymorphism in the 5’UTR region of
DRD4 gene was genotyped by PCR-RFLP method, and the VNTR fragment in exon III of DRD4 gene was investigated by electrophoresis
gel on acrylamide gel method. After eight weeks from the start of drug treatment with methylphenidate, the intensity of symptoms
was evaluated using the Conners scale. Finally, all data from questionnaires and information that were resulted from laboratory
findings were analyzed using ANOVA and repeated measure analysis.
Results: Of the 122 patients under study, 15 patients (12.3%) were responded to the drug treatment, and 107 patients (87.7%) were not
responded. The significant differences were not revealed in genotype, and allele frequencies of between rs3758653 (C/T) and exon III
3’VNTR repeats polymorphisms of the DRD4 gene and responder and non-responder of ADHD groups to the drug treatment.
Conclusions: The results showed that the reduction of ADHD symptoms with drug treatment is not related to DRD4 sub-types in
patients with ADHD.
