Molecular Cloning and Bioinformatic Analysis of Endosperm Specific Promoter, α-Globulin (AsGlo1)

The α-globulin promoter (AsGlo1) have been successfully amplified from pmCACA:GFP using PCR. The construction of the recombinant plasmid, pMRGpro containing α-globulin promoter was achieved by ligation at HindIII and BamHI of pMR104a. Cassette containing α-globulin promoter and nos terminator was successfully cloned in pCAMBIA1305.2 and designated as pCAMGpro. Digestion methods were used to confirm the recombinant plasmid. The resulting plasmid was then sequenced and analyzes using bioinformatics tools. The analysis showed a 99% homology with A. sativa α-globulin gene promoter (Gene Bank: AY795082.1) and further confirmed using phylogenetic analysis. The result showed a successful cloning of the correct fragment Avena sativa α-globulin promoter into an expression vector.