Optimization of EDTA Exudation Technique for Proteome Study of the Phloem

Phloem proteome study is gaining recognition due to the phloem important function to a plant. Identification of proteins within the phloem with varying function could help understand the regulation of growth and development. EDTA exudation technique applied to obtain Arabidopsis phloem protein was optimized. It was found that plants grown up until 6 weeks in SD (short day) condition, exposed to 3 days LD (long day) condition, the phloem sap collected in 20 mM EDTA with overnight incubation in LD and precipitated using Amicon filter device gave the optimum amount of protein with good quality. Sufficient amount of protein was obtained using the optimized EDTA exudation technique for protein identification.