Author/Authors :
Ahmad, Irshad Korea Advanced Institute of Science and Technology - Department of Biological Sciences, Republic of Korea , Khan, Samiullah University of the Punjab - National Center of Excellence in Molecular Biology, Pakistan , Javed, Muhammad Arshad University Technology Malaysia - Faculty of Biosciences and Bioengineering, Malaysia , Fahrul, Zaman Huyopc, Universiti Teknologi Malaysia - Faculty of Biosciences and Medical Engineering, Malaysia , Tariq, Muhammad Universiti Teknologi - Faculty of Biosciences and Medical Engineering, Malaysia , Nasir, Idrees Ahmad University of the Punjab - National Centre of Excellence in Molecular Biology, Pakistan
Abstract :
Genomic DNA of the mutant lines of the three potato cultivars, Cardinal, Diamant and Desiree, with respect to controls were isolated and analyzed for polymorphisms by using random amplified polymorphic DNA (RAPD) markers. Four 10 bp random fragment primers, S-13, S-18, S-19 and R-17 were studied and all of them gave the amplification of genomic DNA. All of the mutant lines gave different banding pattern against different primers with respect to control plants of the three varieties, and bands are present at 50 bp to 1500 bp. All these primers with specific banding pattern were unique in their polymorphic behavior. Different banding pattern of total protein contents were also observed by PAGE analysis of all the mutant lines as compared with the control plants. It is therefore suggested that RAPD and protein analyses would be important tools to detect the polymorphism in mutated lines of potato.
Keywords :
Potato , RAPD , polymorphism , PAGE , mutation
