Title of article :
The Effect of Different Concentrations of Methylprednisolone on Survival, Proliferation, and Migration of Neural Stem/Progenitor Cells
Author/Authors :
Bagheri ، Zohreh Department of Physiology - School of Medicine - Shiraz University of Medical Sciences , Shamsi ، Fatemeh Department of Neurosciences - School of Advanced Medical Sciences and Technologies, Neuroscience Laboratory (Brain, Cognition and Behavior) - Shiraz University of Medical Sciences , Zeraatpisheh ، Zahra Department of Neurosciences - School of Advanced Medical Sciences and Technologies, Neuroscience Laboratory (Brain, Cognition and Behavior) - Shiraz University of Medical Sciences , Salmannejad ، Mahin Department of Anatomy - School of Medicine - Shiraz University of Medical Sciences , Soltani ، Ahmad Department of Neurosurgery - Namazi Hospital - Shiraz University of Medical Sciences , Aligholi ، Hadi Department of Neurosciences - School of Advanced Medical Sciences and Technologies, Neuroscience Laboratory (Brain, Cognition and Behavior), Epilepsy Research Center - Shiraz University of Medical Sciences
Abstract :
Introduction: The present study addressed whether methylprednisolone (MP) as an antiinflammatory drug used in neurodegenerative diseases and neural stem/progenitor cells (NS/PCs) is safe. Methods: First, embryonic rat NS/PCs were exposed to different concentrations of MP, and then we evaluated their survival by MTT assay, proliferation by analyzing the number and diameter of neurospheres, and the migration of the cells by neurosphere assay. Results: The viability of NS/PCs was reduced following exposure to 10, 15, and 20 micro;g/mL of MP. In addition, although the number of neurospheres did not change, exposure to different concentrations of MP resulted in the formation of smaller neurospheres. Despite these undesirable effects, the highest concentration of MP (20 mu;g/mL) increased the migration capacity of the NS/PCs. Conclusion: The combination of MP and NS/PCs is not recommended due to the adverse effects of MP on the survival and proliferation of NS/PCs.
Keywords :
Methylprednisolone , Neural stem cells , Proliferation , Survival , Migration
Journal title :
Basic and Clinical Neuroscience
Journal title :
Basic and Clinical Neuroscience
