Title of article :
Enzymatic Synthesis of Amoxicillin with Immobilized Penicillin G Acylase
Author/Authors :
Alemzadeh, I. sharif university of technology - Deparimeni of Chemical and Petroleum Engineering, تهران, ايران , Borghei, G. sharif university of technology - Department of Chemical and Petroleum Engineering, تهران, ايران , Vafi, L. sharif university of technology - Department of Chemical and Petroleum Engineering, تهران, ايران , Roostaazad, R. sharif university of technology - Department of Chemical and Petroleum Engineering, تهران, ايران
Abstract :
The synthesis of amoxicillin with immobilized penicillin G acylase (PGA) in aqueous medium was investigated. The parameters studied were: time course of amoxicillin production, concentration of substrates: hydroxyphenylglycine methyl ester (HPGM) and 6-aminopeicillanic acid (6- APA) and the effect of enzyme (PGA) content and pH, under variable and constant conditions and temperature variations. In the study of two substrate concentration on amoxicillin production, impressive results were obtained for a 1/3 ratio of 6-amino penicillanic acid (6-APA) and hydroxyl-phenylglycine methyl ester (HPGM). The synthesis of amoxicillin was preferable at constant pH rather than a variable one. Other optimal conditions obtained were: enzyme concentration: 5 g/L with 100U, process time: 480 min and temperature: 35 C. The yield for amoxicillin synthesis under prescribed conditions showed up to 50%.
Keywords :
Amoxicillin , Penicillin G acylase , Aqueous medium , Enzyme content.
Journal title :
Scientia Iranica(Transactions B:Mechanical Engineering)
Journal title :
Scientia Iranica(Transactions B:Mechanical Engineering)
