Author/Authors :
YASMEEN, FARZANA Shalamar Hospital and Shalamar Medical and Dental College, Pakistan , YASMEEN, FARZANA University of Health Sciences - Department of Pathology, Pakistan , MUMTAZ, ASIM Shalamar Hospital and Shalamar Medical and Dental College, Pakistan , MUMTAZ, ASIM University of Health Sciences - Department of Pathology, Pakistan , ADHAMI, SALEEM-UZ-ZAMAN Shalamar Hospital and Shalamar Medical and Dental College, Pakistan , ADHAMI, SALEEM-UZ-ZAMAN University of Health Sciences - Department of Pathology, Pakistan , QURESHI, SAJID A. University of Health Sciences - Department of Pathology, Pakistan , QURESHI, SAJID A. Shalamar Hospital and Shalamar Medical and Dental College, Pakistan
Abstract :
Objective: The objective of this study was to compare and correlate the analytical performance of D10 Hemoglobin Testing System based on Cation Exchange HPLC and Roche Hitachi 902 Immu- noturbidimetric method. Subjects and Methods: A total of 110 patients of Type 2 Diabetes Mellitus were included in the stu- dy. HbA1c was determined using D-10 Hemoglobin Testing system and Roche Hitachi 902 Analy- zer. Results: Both methods showed good correlation with the correlation coefficient (r) of 0.95. Bet- ween run Coefficient of variance was found to be lower for HPLC system compared to immunotur- bidimtric method. HPLC method also produces a chromatogram that shows the different hemoglo- bin fractions, allowing identification of different hemoglobin variants. Conclusion: In the present study both methods showed good correlation but the D10 HPLC system provided adequate throughput and improved precision as compared to immunoturbidimetric me- thod.
Keywords :
HbA1c , Diabetes mellitus , Cation exchange HPLC , Immunoturbidimetry.
