Author/Authors :
aliakbari, f. semnan university of medical sciences - school of medicine - student research committee, department center for biotechnology research, ايران , aliakbari, f. national institute of genetic engineering and biotechnology - institute of industrial and environmental biotechnology - bioprocess engineering department, ايران , shabani, a.a. semnan university of medical sciences - school of medicine - department center for biotechnology research, ايران , bardania, h. yasuj university of medical sciences - cellular and molecular research center, ايران , eslampanah seyedi, h.a. national institute of genetic engineering and biotechnology - institute of industrial and environmental biotechnology - bioprocess engineering department, ايران , mohammad-beigi, h. aarhus university - interdisciplinary nanoscience centre (inano) - department of molecular biology and genetics, Denmark , tayaranian marvian, a. national institute of genetic engineering and biotechnology - institute of industrial and environmental biotechnology - bioprocess engineering department, ايران , nasouti, m. national institute of genetic engineering and biotechnology - institute of industrial and environmental biotechnology - bioprocess engineering department, ايران , vafaei, a.a. semnan university of medical sciences - research center, school of medicine - department of physiology, laboratory of learning and memory, ايران , shojaosadati, s.a. tarbiat modares university - faculty of chemical engineering - biotechnology group, ايران , saboury, a.a. university of tehran - institute of biochemistry and biophysics (ibb), تهران, ايران , christiansen, g. aarhus university - department of biomedicine, Denmark , morshedi, d. national institute of genetic engineering and biotechnology - institute of industrial and environmental biotechnology - bioprocess engineering department, ايران
Abstract :
Alpha-synuclein (ce-Syn) is a protein with high tendency to convert into toxic aggregates that are involved in Parkinson s Disease (PD). The conversion of ce-Syn into toxic aggregates may associate with bio-membranes and the interaction between ce-Syn and bio-mimic liposomes can trigger the neurotoxicity. Some inhibitors of ce-Syn aggregation are challengeable drugs. Using nano carriers such as nanoliposomes may overcome such challenges. Nevertheless, there are few studies on nanoliposomes effects on neuronal cells and ce-Syn. Herein, we investigated the neurotoxicity and fibrinogenesis of neutral charged nanoliposomes. Thin layer evaporation method and hydration method was employed to formulate the nanoliposomes from Dipalmitoylphosphatidylcholine to the size of 100 nm. Fibrillation and cytotoxicity of ce-Syn treated with nanoliposome were measured. Neither neurotoxicity nor fibrillation was observed when ce-Syn treated with different concentrations of nanoliposome. These results well indicate that nanoliposome at the concentrations required for drug delivery not only is able to prevent fibrillation process, but also has no considerable toxic effects on the cells.
Keywords :
Alpha , synuclein , Amyloid , related diseases , Fibrillation , Nanoliposome , Neurotoxicity , Parkinson s disease
