A case control study to determine N-terminal pro- B-type natriuretic peptide levels in patients of heart failure combined with diabetes

Heart failure (HF) is a disorder in which the heart muscle becomes weaker than normal. This condition occurrence increases when patient has previous diagnosis of diabetes mellitus (DM). This study focused on determination of NT-proBNP biomarker and many routine parameters in HF patients that already have DM. 120 blood samples from males and females (80 patients and 40 controls) were collected. The patients and controls were all between the ages of 40 and 70. Patients’ samples were taken from Ibn Al-Bitar Diagnostic Center for Heart Surgery in Baghdad/ Iraq. Serum levels of sugar, total cholesterol, HDL cholesterol, LDL cholesterol, and triglycerides were calculated spectrophotometrically. Glycated hemoglobin (HbA1C) level was determined using biochemical kit. Finally, NT-proBNP and insulin levels were measured using ELISA kits. Serum levels of glucose, HbA1C, insulin, and NT-proBNP were significantly higher (p=0.000) and there are significant differences between patients and control groups. Lipid profile parameters except cholesterol and SLDL have a significant difference. NT-proBNP was negatively correlated to glucose concentration, whereas it was positively correlated to heart failure and insulin concentration. In Cluster analysis, NT-proBNP was organized in one cluster with SLDL. The best cutoff value for NT-proBNP protein was (54.1) ng/mL according to ROC analysis.