Author/Authors :
Yousuf, D.A. Tanta University - Faculty of Dentistry - Department of Oral Medicine, Periodontology, Oral Diagnosis Oral Radiology, Egypt , Afify, O.M. Tanta University - Faculty of Dentistry - Department of Oral Medicine, Periodontology, Oral Diagnosis Oral Radiology, Egypt , El Soudany, K.S. Tanta University - Faculty of Dentistry - Department of Oral Medicine, Periodontology, Oral Diagnosis Oral Radiology, Egypt , Ghoniem, S.M. Tanta University - Faculty of Dentistry - Department of Oral Pathology, Egypt
Abstract :
Background: The aim of our study was to evaluate the effect of local application of melatonin gel on the healing of surgically induced periodontal osseous defects in diabetes-induced rabbits. Material and methods: Diabetes induction was done for 28 rabbits, then, acute periodontal osseous defects were done. Rabbits were divided into: group I: periodontal defects filled with collaplug saturated with placebo gel and group II: periodontal defects filled with collaplug saturated with 2 mg melatonin gel. The expression of RANKL m RNA in the gingival biopsies was examined by real time RT-PCR. Histological and histomorphometric evaluation were performed for both groups at 3 and 6 weeks. Results: The levels of RANKL mRNA expression were significantly increased at 2 weeks after diabetes induction. Then, ten days after application of melatonin gel, the level of expression of RANKL significantly decreased as compared to control group. Histologically and histomorphometrically, there was a high significant increase in the percentage of new bone formation and the number of osteoblasts in melatonin-treated group as compared to control group. The number of osteoclasts significantly decreased in melatonin-treated group in both examination periods. Conclusion: The local application of 2 mg melatonin gel offers a promising therapeutic approach for an efficient bone and PDL regeneration.
