Author/Authors :
Kumar Agnihotri, Saurabh Department of Radiotherapy - King George’s Medical University - Lucknow 226 003, India - Division of Endocrinology - CSIR-Central Drug Research Institute - Lucknow 226 031, India , Kumar, Balawant Division of Toxicology & Experimental Medicine - CSIR-Central Drug Research Institute - Lucknow 226 031, India , Jain, Ankita Division of Endocrinology - CSIR-Central Drug Research Institute - Lucknow 226 031, India , Anjali, Anjali Division of Toxicology & Experimental Medicine - CSIR-Central Drug Research Institute - Lucknow 226 031, India - Department of Obstetrics & Gynaecology - King George’s Medical University - Lucknow 226 003, India , Pal Singh Negi, Mahendra Division of Toxicology & Experimental Medicine - CSIR-Central Drug Research Institute - Lucknow 226 031, India , Sachan, Rekha Department of Obstetrics & Gynaecology - King George’s Medical University - Lucknow 226 003, India , Brahma Bhatt, Madan Lal Department of Radiotherapy - King George’s Medical University - Lucknow 226 003, India , Tripathi, Raj Kamal Division of Toxicology & Experimental Medicine - CSIR-Central Drug Research Institute - Lucknow 226 031, India - Department of Obstetrics & Gynaecology - King George’s Medical University - Lucknow 226 003, India , Sachdev, Monika Academy of Scientific and Innovative Research (AcSIR) - Ghaziabad 201 002, India - Division of Endocrinology - CSIR-Central Drug Research Institute - Lucknow 226 031, India
Abstract :
Background: This study correlates the serum levels of sCD95 & TNF-α with a simple cell-based assay to
evaluate the capacity of the serum sample to induce apoptosis in Jurkat cells. Interlinking of these parameters
can be explored to design a minimum invasive diagnostic strategy for cervical cancer (CC).
Methods: Sera samples were assessed to induce apoptosis in Jurkat cells through FACS. Serum levels of
sCD95 and TNF-α were measured by ELISA. JNK phosphorylation was evaluated in sera incubated Jurkat
cells. Data was scrutinized through statistical analysis.
Results: Significantly higher serum levels of sCD95 and lower TNF-α levels were observed in CC patients;
their sera samples inhibited induction of apoptosis in Jurkat cells through reduced JNK phosphorylation.
Statistical analysis linked these three parameters for the early screening of CC.
Conclusions: Distinct sera levels of sCD95 & TNF-α in CC patients showed an anti-apoptotic effect, which can be considered for early detection of CC.
Keywords :
Apoptosis , sCD95 , Jurkat Cells , Tumor Necrosis Factor-alpha , Uterine Cervical Neoplasms
