Title of article :
The promoter region (G-800A and C-509T) polymorphisms of transforming growth factor-β1 gene among young women with recurrent urinary tract infection
Author/Authors :
syukri, m. syiah kuala university - school of medicine - department of internal medicine, Indonesia , sja bani, m. gadjah mada university - faculty of medicine - department of internal medicine, Indonesia , soesatyo, m.h.n.e. gadjah mada university - faculty of medicine - department of histology and cell biology, Indonesia , astuti, i. gadjah mada university - faculty of medicine - department of pharmacology, Indonesia , imran, i. syiah kuala university - school of medicine - medical research unit, Indonesia , harapan, h. syiah kuala university - school of medicine - medical research unit, Indonesia
Abstract :
Background: Recurrent urinary tract infection (UTI) is common among young women and one of its risk factors is genetic. Polymorphisms in promoter region (G-800A (rs1800468) and C-509T (rs1800469)) of transforming growth factor-β1 (TGF-β1) gene play pivotal roles in several infection diseases but the association of these polymorphisms with recurrent UTI remains unclear. The aim of this study was to assess the correlation of TGF-β1 G-800A and C-509T polymorphisms with recurrent UTI in young women. Subjects and methods: TGF-β1 G-800A and C-509T polymorphisms among 34 recurrent UTI patients and 34 healthy subjects,aged 15-50. years old,were evaluated with polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and confirmed by DNA sequencing. Results: At position -800,genotypes showed no significant differences between recurrent UTI patients (GG 97.1%; GA 2.9%; AA 0%) and normal control (GG 97%; GA 0%; AA 2.9%) young women. Dominant and recessive model analyses did not find significant correlation between recurrent UTI patients and normal control young women. At position -509,allelic and genotypic frequencies showed no significant differences between recurrent UTI patients (CC 20.6%; CT 61.8%; TT 17.7%) and control individuals (CC 2.9%; CT 73.6%; TT 23.5%). Conclusion: This study found that there is no strong correlation between polymorphisms of TGF-β1 G-800A and C-509T and recurrent UTI. © 2014 .
Keywords :
Polymorphism , Recurrent UTI , SNP , TGF , β1 , Transforming growth factor , Urinary tract infection
Journal title :
Egyptian Journal of Medical Human Genetics
Journal title :
Egyptian Journal of Medical Human Genetics