Author/Authors :
Chen, Y.T Institute of Biomedical Engineering - College of Biomedical Engineering - Taiyuan University of Technology - Taiyuan, Shanxi, China - Shanxi Key Laboratory of Material Strength & Structural Impact - Taiyuan University of Technology - Taiyuan, Shanxi, China - Research Center for Nanobiomaterials & Regenerative Medicine - College of Biomedical Engineering - Taiyuan University of Technology - Taiyuan, Shanxi, China , Li, C.C Institute of Biomedical Engineering - College of Biomedical Engineering - Taiyuan University of Technology - Taiyuan, Shanxi, China - Shanxi Key Laboratory of Material Strength & Structural Impact - Taiyuan University of Technology - Taiyuan, Shanxi, China - Research Center for Nanobiomaterials & Regenerative Medicine - College of Biomedical Engineering - Taiyuan University of Technology - Taiyuan, Shanxi, China , Liu, Y Institute of Biomedical Engineering - College of Biomedical Engineering - Taiyuan University of Technology - Taiyuan, Shanxi, China - Shanxi Key Laboratory of Material Strength & Structural Impact - Taiyuan University of Technology - Taiyuan, Shanxi, China - Research Center for Nanobiomaterials & Regenerative Medicine - College of Biomedical Engineering - Taiyuan University of Technology - Taiyuan, Shanxi, China , Zheng, Y.Q Centre of Radiation Oncology - Shanxi Cancer Hospital - Taiyuan, Shanxi, China , Yang, X.H Shanxi Provincial Cancer Institute Laboratory Animal Center - Shanxi Cancer Hospital - Taiyuan, Shanxi, China , Yang, C.X Institute of Biomedical Engineering - College of Biomedical Engineering - Taiyuan University of Technology - Taiyuan, Shanxi, China - Shanxi Key Laboratory of Material Strength & Structural Impact - Taiyuan University of Technology - Taiyuan, Shanxi, China - Research Center for Nanobiomaterials & Regenerative Medicine - College of Biomedical Engineering - Taiyuan University of Technology - Taiyuan, Shanxi, China , An, M.W Institute of Biomedical Engineering - College of Biomedical Engineering - Taiyuan University of Technology - Taiyuan, Shanxi, China - Shanxi Key Laboratory of Material Strength & Structural Impact - Taiyuan University of Technology - Taiyuan, Shanxi, China - Research Center for Nanobiomaterials & Regenerative Medicine - College of Biomedical Engineering - Taiyuan University of Technology - Taiyuan, Shanxi, China
Abstract :
Background: Radiation not only kills tumor cells, but also damages other sites. The mechanisms of damage caused by the bystander effect of irradiation in animal models are unclear and the time node is single. In this study, we aimed to investigate the inflammatory response of thymus tissue
injury in non-irradiated areas at different times after irradiating rat skin.
Materials and Methods: Rats were irradiated with an X-ray dose of 38 Gy,
and at 15 d after irradiation, when the skin wound was most severe, the proinflammatory
drug high mobility group box1 (HMGB1) and the antiinflammatory
drug glycyrrhizic acid (GA) were injected intraperitoneally into
rats. After irradiation, skin tissues were collected for histology, and thymus
tissues were collected for gene and protein testing. Results: Animal model of
skin damage was successfully established. The expression of macrophage
(F4/80) increased after irradiation, and F4/80 produced cytokines. Through
the flow which was activated by inflammatory factors in the blood, DNA
damage and the expression of inflammatory-related cytokines in nonirradiated
area of the thymus peaked at 15 d after irradiation. Moreover,
HMGB1 treatment increased the expression at 1 d after intraperitoneal
injection, and GA solution decreased the expression of inflammatory-related
cytokines. Conclusion: When radiation damages the skin, it can cause damage
to other organs through the circulation, and an anti-inflammatory GA solution
reduced inflammatory responses, which are required to modify radiationinduced
systemic effects with anti-inflammatory drugs or agents that affect pathways that cause bystander instability.
Keywords :
Radiation induced bystander effects , thymus , γH2AX , NLRP3 , HMGB1
