Author/Authors :
Vafaee, Reza Proteomics Research Center - Faculty of Paramedical Sciences - Shahid Beheshti University of Medical Sciences, Tehran, Iran , Nikzamir, Abdolrahim Faculty of Medicine - Shahid Beheshti University of Medical Sciences, Tehran, Iran , Razzaghi, Mohhamadreza Laser Application in Medical Sciences Research Center - Shahid Beheshti University of Medical Sciences, Tehran, Iran , Rezaei Tavirani, Sina Gastroenterology and Liver Diseases Research Center - Research Institute for Gastroenterology and Liver Diseases - Shahid Beheshti University of Medical Sciences, Tehran, Iran , Ahmadzadeh, Alireza Faculty of Medicine - Shahid Beheshti University of Medical Sciences, Tehran, Iran , Emamhadi, MohammadAli Shahid Beheshti Medical University, Tehran, Iran
Abstract :
Introduction: Genomics and bioinformatics are useful methods for exploring unclear aspects of
radiation effects on biological systems. Many radiation-induced alterations in irradiated samples are
post-radiation time-dependent. This study aims to evaluate the post-irradiation effects of the gamma
ray on human Jurkat cells.
Methods: Gene expression profiles of the samples harvested 6 and 24 hours after radiation to find
the critical differential expressed genes and the related pathways. Samples are provided from Gene
Expression Omnibus (GEO) and analyzed by ClueGO.
Results: Twnety-nine critical genes were determined as the important affected genes and 7 classes
of related pathways were introduced. CCNE2, PSMD11, CDC25C, ANAPC1, PLK1, AURKA, and
CCNB1 that were associated with more than 6 pathways were related to one of the determined
pathway groups.
Conclusion: Cell protecting pathways were associated with the genes (HSPA5, HSPA8, HSP90B1,
HMMR, CEBPB, RXRA, and PSMD11) which were related to the minimum numbers of pathways.
The finding of this study corresponds to repair processes which depend on post-radiation time. It
seems these sets of genes are suitable candidates for further investigation.
Keywords :
Radiation , Bioinformatics , Gene expression , Pathway , Dysregulation
