Author/Authors :
Razzaghi, Mohammad Reza Laser Application in Medical Sciences Research Center - Shahid Beheshti University of Medical Sciences, Tehran, Iran , Ghazimoradi, Mohammad Hossein Department of Cell and Molecular Biology - Faculty of Life Sciences and Biotechnology - Shahid Beheshti University, Tehran, Iran , Afzali, Shervin Department of Cell and Molecular Biology - Faculty of Life Sciences and Biotechnology - Shahid Beheshti University, Tehran, Iran , Kamani, Ehsan Laser Application in Medical Sciences Research Center - Shahid Beheshti University of Medical Sciences, Tehran, Iran , Mohajerani, Ezeddin POMP Lab - Photonics Department - Laser and Plasma Research Institute - Shahid Beheshti University, Tehran, Iran , Shirkavand, Afshan POMP Lab - Photonics Department - Laser and Plasma Research Institute - Shahid Beheshti University, Tehran, Iran , Farivar, Shirin Department of Cell and Molecular Biology - Faculty of Life Sciences and Biotechnology - Shahid Beheshti University, Tehran, Iran
Abstract :
Introduction: The cytotoxicity of chemotherapy drugs is a significant challenge in the way of
surmounting cancer. Liposomal drug delivery has proven to be efficacious in increasing the function
of the drugs. Its potential to accumulate drugs in the target site and enhance the efficiency of anti-
cancer agents with lower doses hinders their cytotoxicity on normal healthy cells. Since the release
of drugs from liposomes is not generally on a controlled basis, several studies have suggested that
external stimuli including lasers could be used to induce controlled release and boost the efficiency
of liposomal drug delivery systems (LDDSs).
Methods: The A375 cancer cell line was used and exposed to the liposomes containing doxorubicin
in the presence of a low-level laser beam to investigate its effect on the liposomal stimuli-
responsiveness release and its toxicity on cancer cells. So as to achieve that goal, Annexin V/PI was
employed to analyze the number of cells that underwent apoptosis and necrosis.
Results: Here, we report the effect of laser irradiation on LDDSs. According to the results obtained
from the annexin V/PI assay, the pattern of viability status has shifted, so that the number of pre-
apoptotic cells treated with liposomal doxorubicin and a laser beam was more than that of cells
treated with only liposomal doxorubicin.
Conclusion: The use of stimuli-responsive LDDSs, in this case, laser-responsive, has led to favorable
circumstances in the treatment of cancer, offering enhanced cancer cell cytotoxicity.
Keywords :
Low-level laser , Stimuli-sensitive liposomes , Cancer cytotoxicity , Liposomal drug delivery , Apoptosis
