Author/Authors :
Gazor, Rouhallah Department of Anatomy and Cell Biology - Faculty of Medicine - Guilan University of Medical Sciences, Rasht, Iran , Asgari, Mehrdad Department of Anatomy and Cell Biology - Faculty of Medicine - Guilan University of Medical Sciences, Rasht, Iran , Abdollajhifar, Mohammad-Amin Department of Biology and Anatomical Sciences - School of Medicine - Shahid Beheshti University of Medical Sciences, Tehran, Iran , Kiani, Pejman Department of Neuroscience and Addiction Studies - School of Advanced Technologies in Medicine - Tehran University of Medical Science, Tehran, Iran , Zare, Fatemeh Department of Biology and Anatomical Sciences - School of Medicine - Shahid Beheshti University of Medical Sciences, Tehran, Iran , Fadaei Fathabady, Fatemeh Department of Biology and Anatomical Sciences - School of Medicine - Shahid Beheshti University of Medical Sciences, Tehran, Iran , Norouzian, Mohsen Department of Biology and Anatomical Sciences - School of Medicine - Shahid Beheshti University of Medical Sciences, Tehran, Iran , Amini, Abdollah Department of Biology and Anatomical Sciences - School of Medicine - Shahid Beheshti University of Medical Sciences, Tehran, Iran , Khosravipour, Armin Department of Biology and Anatomical Sciences - School of Medicine - Shahid Beheshti University of Medical Sciences, Tehran, Iran , Atashgah, Rahimeh B. Department of Pharmaceutical Biomaterials - Faculty of Pharmacy - Tehran University of Medical Sciences, Tehran, Iran , Kazemi, Mahsa Department of Biology and Anatomical Sciences - School of Medicine - Shahid Beheshti University of Medical Sciences, Tehran, Iran , Chien, Sufan Price Institute of Surgical Research - University of Louisville, Kentucky, USA , Bayat, Mohammad Department of Biology and Anatomical Sciences - School of Medicine - Shahid Beheshti University of Medical Sciences, Tehran, Iran
Abstract :
Introduction: The ability of simultaneous treatment of critical-sized femoral defects (CSFDs) with photobiomodulation (PBM) and demineralized bone matrix (DBM) with or without seeded adipose-derived stem cells (ASCs) to induce bone reconstruction in ovariectomized induced osteoporotic (OVX) rats was investigated.
Methods: The OVX rats with CSFD were arbitrarily separated into 6 groups: control, scaffold (S, DBM), S + PBM, S + alendronate (ALN), S + ASCs, and S + PBM + ASCs. Each group was assessed by cone beam computed tomography (CBCT) and histological examinations.
Results: In the fourth week, CBCT and histological analyses revealed that the largest volume of new bone formed in the S + PBM and S + PBM + ASC groups. The S + PBM treatment relative to the S and S + ALN treatments remarkably reduced the CSFD (Mann-Whitney test, P = 0.009 and P = 0.01). Furthermore, S + PBM + ASCs treatment compared to the S and S + ALN treatments significantly decreased CSFD (Mann Whitney test, P = 0.01). In the eighth week, CBCT analysis showed that extremely enhanced bone regeneration occurred in the CSFD of the S + PBM group. Moreover, the CSFD in the S + PBM group was substantially smaller than S, S + ALN and S + ASCs groups (Mann Whitney test, P = 0.01, P = 0.02 and P = 0.009). Histological observations showed more new bone formation in the treated CSFD of S + PBM + ASCs and S + PBM groups.
Conclusion: The PBM plus DBM with or without ASCs significantly enhanced bone healing in the CSFD in OVX rats compared to control, DBM alone, and ALN plus DBM groups. The PBM plus DBM with or without ASCs significantly decreased the CSFD area compared to either the solo DBM or ALN plus DBM treatments.
