Title of article :
Antibacterial Effect of Combination of Cinnamon Essential Oil and Thymol, Carvacrol, Eugenol, or Geraniol
Author/Authors :
El Atki, Yassine Department of Biology - Laboratory of Physiology Pharmacology and Environmental Health - Faculty of Sciences Dhar El Mehraz - Sidi Mohamed Ben Abdellah University, Fez, Morocco , Aouam, Imane Department of Biology - Laboratory of Physiology Pharmacology and Environmental Health - Faculty of Sciences Dhar El Mehraz - Sidi Mohamed Ben Abdellah University, Fez, Morocco , Taroq, Amal Department of Biology - Laboratory of Physiology Pharmacology and Environmental Health - Faculty of Sciences Dhar El Mehraz - Sidi Mohamed Ben Abdellah University, Fez, Morocco , El Kamari, Fatima Department of Biology - Laboratory of Physiology Pharmacology and Environmental Health - Faculty of Sciences Dhar El Mehraz - Sidi Mohamed Ben Abdellah University, Fez, Morocco , Timinouni, Mohammed Molecular Bacteriology Laboratory - Pasteur Institute of Morocco, Casablanca , Lyoussi, Badiaa Department of Biology - Laboratory of Physiology Pharmacology and Environmental Health - Faculty of Sciences Dhar El Mehraz - Sidi Mohamed Ben Abdellah University, Fez, Morocco , Abdellaoui, Abdelfattah Department of Biology - Laboratory of Physiology Pharmacology and Environmental Health - Faculty of Sciences Dhar El Mehraz - Sidi Mohamed Ben Abdellah University, Fez, Morocco
Abstract :
Bacterial resistance to classic antibiotics is an alarming rate to put this into control with the use of
natural products of plant derivatives. The objective of this study was to determine the phytochemical of
cinnamon essential oil (EO) and to evaluate its antibacterial activity alone and in combination with some
main components of EOs such as thymol, carvacrol, eugenol, or geraniol against three bacterial strains
(Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa). The phytochemical analysis of
cinnamon EO was evaluated using gas chromatography-flame ionization detector and gas chromatographymass
spectrometer analysis. The antibacterial activity of tested compounds was determined by agar disk
diffusion and minimum inhibitory concentration (MIC) assays. The checkerboard method was used to
quantify the efficacy of cinnamon EO in combination with those compounds. The results showed that
the major compound in the cinnamon EO was trans-cinnamaldehyde (91.01%). Cinnamon oil was the
highest antibacterial activity with MIC of 0.005, 0.005, and 0.02 mg/mL against E. coli, S. aureus, and
P. aeruginosa, respectively. Synergistic activity was shown only against S. aureus by the combination of
cinnamon EO and thymol. The additive effect was found against E. coli when cinnamon EO was combined
with thymol or carvacrol, and against S. aureus when cinnamon EO was combined with carvacrol. However,
the combination of EO and thymol or carvacrol showed an indifference action against P. aeruginosa. The
combination of cinnamon EO with thymol or carvacrol can be used as an alternative therapeutic agent
for medical application and as a natural preservative.
Keywords :
Antibacterial activity , cinnamon , combination , gas chromatography-mass spectrometer analysis
