Author/Authors :
Abbasi, Abbas Faculty of Nutrition and Food Industry - Pharmaceutical Science Research Center - Kermanshah University of Medical Sciences, Kermanshah, Iran , Azizi, Ali Department of Community Medicine - Faculty of Medicine - Kermanshah University of Medical Sciences, Kermanshah, Iran , Nachvak, mostafa Faculty of Nutrition and Food Industry - Pharmaceutical Science Research Center - Kermanshah University of Medical Sciences, Kermanshah, Iran , Alizadeh, Elaheh Faculty of Nutrition and Food Industry - Pharmaceutical Science Research Center - Kermanshah University of Medical Sciences, Kermanshah, Iran , Abbsavaran, Rezvan Faculty of Nutrition and Food Industry - Pharmaceutical Science Research Center - Kermanshah University of Medical Sciences, Kermanshah, Iran , Mirtaheri, Elham Faculty of Nutrition and Food Industry - Pharmaceutical Science Research Center - Kermanshah University of Medical Sciences, Kermanshah, Iran , Moradinazar, Mehdi Behavioral Disease Research Center - Kermanshah University of Medical Sciences, Kermanshah, Iran , Rahimi, Mehrali Diabetes Research Center - Kermanshah University of Medical Sciences, Kermanshah, Iran
Abstract :
Aim: Ginger is a natural dietary rhizome with antioxidant, anti-inflammatory, and anticarcinogenic
properties. It has many medical beneficial properties such as anti-proliferation and antiapoptotic
effects on cancerous esophageal cells. Materials and Methods: Esophageal cancer cells ESO26 were
cultured in the presence and absence of ginger extract at various concentrations for 12, 18, and 24 h.
Then, the viability was determined by 3-(4,5-dimethylthiazol)-2,5-diphenyl tetrazolium bromide (MTT)
assay. Western blot analysis of caspase-3 was performed to detect apoptosis. p21, Bax, and Bcl-2 gene
expression was measured using quantitative polymerase chain reaction (PCR). Data were analyzed using
one-way analysis of variance (ANOVA) followed by Tukey test. Results: The ginger extract increased the
cleavage of caspase-3 in cells (P < 0.05). Results of real-time PCR have shown that ginger decreased the
expression of Bcl-2 and increased Bax and p21 gene expression (P < 0.05). Conclusions: Results showed
that the process of cell proliferation has been stopped. Also, this study indicated that ginger might exert
a chemopreventive effect on esophageal cancer through the suppression of proliferation and the growth
of tumor cells as well as the induction of apoptosis.
Keywords :
Apoptosis , caspase-3 , esophageal cancer , ginger
