Author/Authors :
Naghizadeh, Sajad Department of Pharmacology and Toxicology - Faculty of Medicine - AJA University of Medical Sciences, Tehran, Iran , Paknejad, Babak Department of Pharmacology and Toxicology - Faculty of Medicine - AJA University of Medical Sciences, Tehran, Iran , Abtahi, Reza Department of Pharmacology and Toxicology - Faculty of Medicine - AJA University of Medical Sciences, Tehran, Iran , Hazrati, Ebrahim Department of Anesthesia and Intensive Care - Faculty of Medicine - AJA University of Medical Sciences, Tehran, Iran , Chamanara, Mohsen Department of Pharmacology and Toxicology - Faculty of Medicine - AJA University of Medical Sciences, Tehran, Iran
Abstract :
A simple free labeled electrochemical immunosensor based on chitosan (CHI) and quantum dots (QDs)
was developed for the determination of procalcitonin (ProCT) antigen (Ag) which can act as a target
biomarker of infectious diseases. The antibodies of the ProCT biomarker were successfully incubated
on the CHI-QDs platform. Electrochemical impedance spectroscopy and cyclic voltammetry were used
for the evaluation and characterization of various layers coated onto the electrode. Under the optimum
conditions, the electron transfer resistance (Rct) of the immunosensor was altered linearly by the ProCT
concentration. The proposed immunosensor was proportional to the ProCT biomarker concentration
from 0.005 to 0.35ng/mL with a calculated detection limit of 0.015 pg/mL. The proposed biosensing
device also exhibited high stability and reproducibility, which can be used for the quantitative assay of
the ProCT biomarker in clinic analyses.
Keywords :
Carbon paste electrode , Chitosan , electrochemical impedance spectroscopy , immunosensor , quantum dots
