Author/Authors :
Hassan, Alaa J. University of Babylon - College of science - Department of biology, Iraq , AL-Alwany, Shakir H. University of Babylon - College of science - Department of biology, Iraq , Rahim, .T.Abbas Babylon Health Office, Iraq , Zahra a, M. A.A University of Babylon - College of science - Department of biology, Iraq , Sada Sadiq, A.O University of Babylon - College of science - Department of biology, Iraq , Lelo, Rafal Ahmed University of Babylon - College of science - Department of biology, Iraq
Abstract :
Staphylococcus aureus was isolated from patients of Hilla hospital for surgery .The killed antigen of S. aureus was prepared and mixed with incomplete Freund’s adjuvant then injected in rats . The results revealed that the effect of S. aureus antigen in cellular immunity which including ; phagocytosis ,endocytic killing by neutrophils and ingestion of bacteria by phagocytes at different times of incubation in rats primed with bacterial antigen . The means of Phagocytosis were reached 15.2 ، 35.4 ،and 50.7 after 10 ,30 and 50 min of incubation respectively ،while the means of endocytic killing were reached 4.8 ،4.9 ،and 9.8 at the same times mentioned above . Furthere more ،the rates of ingestion were 0.48 ,0.178 and 0.196 at the same times respectively،while lower in above tests in control rats . The concentrations of total protein ،albumin and crude globulins were appeared lower in treated rats compared with control animals ،which reached 9.45 ,8.02 and 1.45 g/L, while it was 12.34 ,10.44 and 1.9 g/L for control group respectively .These results suggests that the killed antigen of S. aureus stimulated the cellular immunity ،while it was suppressive for humoral immunity in rats.
