Title of article :
In vitro evaluation of hydroalcoholic extracts of Capparis spinosa L., Ricinus communis, and Solanum luteum on Leishmania major (MRHO/IR/75/ER) promastigotes
Author/Authors :
Zadeh-Abbasi Zarandi, Hedieh Department of Agricultural Biotechnology - Faculty of Agriculture - Shahid Bahonar University of Kerman, Kerman, Iran , Shirani-Bidabadi, Leila Department of Vector Biology and Control - School of Public Health - Kerman University of Medical Sciences, Kerman, Iran - Research Center of Tropical and Infectious Diseases Kerman University of Medical Sciences, Kerman, Iran , Aghaei-Afshar, Abass Leishmaniasis Research Center - Kerman University of Medical Sciences, Kerman, Iran , Eghbalian, Mostafa Department of Biostatistics and Epidemiology - School of Public Health - Kerman University of Medical Sciences, Kerman, Iran , Zolala, Jafar Department of Agricultural Biotechnology - Faculty of Agriculture - Shahid Bahonar University of Kerman, Kerman, Iran , Mirtadjadini, Mansour Department of Agricultural Biotechnology - Faculty of Agriculture - Shahid Bahonar University of Kerman, Kerman, Iran , Saghafipour, Abedin Department of Public Health - Faculty of Health - Qom university of Medical Sciences, Qom, Iran , Salarkia, Ehsan Leishmaniasis Research Center - Kerman University of Medical Sciences, Kerman, Iran
Abstract :
Background: Plant extracts or compounds derived from plants are a valuable source for finding new anti-leishmaniasis drugs.
Objective: In this study, Capparis spinosa L., Ricinus communis, and Solanum luteum were used as lethal agents for the promastigotes
of Leishmania major parasites in the culture medium.
Methods: Diluted extracts of 12.5, 100, and 500 mg/mL were prepared from medicinal plant dried extracts. Glucantime at a concentration
of 500 mg/mL was used as a positive control.
Results: For all three extracts, concentrations of 100 and 500 mg/mL could kill promastigotes at higher rates and speeds compared
to other concentrations. The high concentrations of extracts (500 mg/mL) presented similar effects. According to the findings,
hydroalcoholic extract of C. spinosa L. presented considerably lower antiparasitic effects, and S. luteum and R. communis extracts
were could kill most of the parasitic promastigotes at higher doses. TheANOVA test did not show any significant viability percentage
difference of Leishmania extracts between different extract types.
Conclusions: In this study, the lethal effects of R. communis and S. luteum hydroalcoholic extracts on L. major promastigotes were
found to be stronger than the C. spinosa L. extract.
Keywords :
Capparis spinosa L. , Ricinus communis , Solanum luteum , Leishmania major , Promastigotes , in vitro
