Author/Authors :
Zaker, Rasoul Department of Biology - Faculty of Sciences -University of Isfahan, Isfahan, Iran , Gavanji. Shahin Young Researchers and Elite Club - Isfahan (Khorasgan) Branch Islamic Azad University, Isfahan, Iran , Sayedipour. Sana Department of Biotechnology - Faculty of Advanced Sciences and Technologies - University of Isfahan, Isfahan, Iran , Bakhtari, Azizollah Department of Animal Sciences - College of Agriculture - Isfahan University of Technology, Isfahan, Iran , Shirani Bidabadi. Elham epartment of Biotechnology - Faculty of Advanced Sciences and Technologies - University of Isfahan, Isfahan, Iran , Larki, Behrouz Young Researchers and Elite Club - Isfahan (Khorasgan) Branch Islamic Azad University, Isfahan, Iran , Golestannejad, Zahra OralMedicine Department - Dental School and Torabinejad Research Center, Isfahan, Iran
Abstract :
Nowadays, many antibiotics are being used to control infectious diseases. Inappropriate
use of antibiotics leads to emergence of resistant bacteria and problems such as a
prolonged course of treatment. Medicinal plants and their derivatives, as a good source
of treatment, are effective against drug resistance In this study we have evaluated the
antimicrobial activity of herbal essential oils of four medicinal plant Cuminum
cyminum, Satureja hotensis L, Citrus limon and Mentha piperita. For preparation of
herbal essential oils clevenger apparatus was used, and the active components of the
herbal essential oils were determined by GC-MS. In order to observe the effects of the
essential oils, they were examined on Staphylococcus aureus, Escherichia coli and
Salmonella typhi using disc diffusion method in vitro. Also, the Minimal Inhibitory
Concentration (MIC) and Minimal Bactericide Concentration (MBC) of each essential
oil was measured and compared with commonly used antibiotics. Obtained results
showed that all of the essential oils possess inhibitory and antibacterial effects, but the
Mentha piperita essential oil showed a better effect in comparison to other used
essential oils. It was concluded that all the herbal species demonstrate antibacterial
properties, but the level of bacterial growth inhibition induced by plant materials, shown
to be dependent on herbal source and bacterial strain.
Keywords :
Herbal essential oils , GC-MS , Minimal Inhibitory Concentration , Minimal Bactericide Concentration