Title of article :
Study of The Correlation between miR-106a, miR-125b, and miR-330 on Multiple Sclerosis Patients by Targeting TNFSF4 and SP1 in NF-кb/TNF-α Pathway: A Case-Control Study
Author/Authors :
Hadi ، Nasrin Medical Biotechnology Research Center - Islamic Azad University, Ashkezar Branch , Seifati ، Morteza Medical Biotechnology Research Center - Islamic Azad University, Ashkezar Branch , Nateghi ، Behnaz Medical Genetics Research Center of Genome, Cellular, Molecular, and Genetics Research Center - Isfahan University of Medical Sciences , Ravaghi ، Parisa Department of Biology - Faculty of Science - Shahid Chamran University of Ahvaz , Khosravian ، Farinaz Medical Genetics Research Center of Genome, Cellular, Molecular, and Genetics Research Center - Isfahan University of Medical Sciences , Namazi ، Faezeh Medical Biotechnology Research Center - Islamic Azad University, Ashkezar Branch , Fotouhi Firouzabad ، Maryam Medical Biotechnology Research Center - Islamic Azad University, Ashkezar Branch , Shaygannejad ، Vahid Department of Neurology - Isfahan Neurosciences Research Center - Isfahan University of Medical Sciences , Salehi ، Mansoor Department of Genetics and Molecular Biology - Medical Genetics Research Center of Genome, Cellular, Molecular, and Genetics Research Center, School of Medicine - Isfahan University of Medical Sciences
Abstract :
Objective: Multiple sclerosis (MS) is a complex multifactorial neuro-inflammatory disorder. This complexity arises from the evidence suggesting that MS is developed by interacting with environmental and genetic factors. This study aimed to evaluate the miR-106a, miR-125b, and miR330- expression levels in relapsing-remitting multiple sclerosis (RRMS) patients. The miRNAs impact on TNFSF4 and Sp1 genes through the NF-кB/TNF-α signaling pathway was analyzed by measuring the expression levels in case and controls. Materials and Methods: In this in silico-experimental study, we evaluated the association of miR-106a, miR-125b, and miR330- with TNFSF4 and SP1 gene expression levels in 60 RRMS patients and 30 healthy controls by real-time polymerase chain reaction (PCR). Results: The expression levels of miR-330, miR-106a, and miR125-b in blood samples of RRMS patients were predominantly reduced. The expression of TNFSF4 in patients demonstrated a significant enhancement, in contrast to the diminishing Sp1 gene expression level in controls. Conclusion: Our findings indicated an association between miR-106a and miR-330 and miR125-b expression and RRMS in our study population. Our data suggested that the miR106-a, miR125-b, and mir330- expression are correlated with TNFSF4 and Sp1 gene expression levels.
