Author/Authors :
Feyzmanesh ، Somayeh Department of Anatomical Sciences - Faculty of Medical Sciences - Tarbiat Modares University , Halvaei ، Iman Department of Anatomical Sciences - Faculty of Medical Sciences - Tarbiat Modares University , Baheiraei ، Nafiseh Department of Anatomical Sciences, Tissue Engineering and Applied Cell Sciences Division - Faculty of Medical Sciences - Tarbiat Modares University
Abstract :
Objective: The main goal was to evaluate the effects of alginate on human sperm parameters during cryopreservation. Materials and Methods: In this prospective study, twenty-five normozoospermic samples were divided into two groups, encapsulated with 1% alginate and the control group. The samples were then frozen by rapid freezing. Different sperm parameters including motility, normal morphology, viability, acrosome reaction, and DNA integrity, were examined before freezing and after thawing. Results: All sperm parameters had a significant decrease after thawing compared to before freezing. Our data showed a significant decrease in sperm motility of the alginate group but sperm viability, normal morphology, and DNA fragmentation were similar between the two groups. However, the rates of intact acrosome and native DNA were significantly lower in the control group compared to the alginate group (45.12 ± 11.1 vs. 55.25 ± 10.69 and 52.2 ± 11.92 vs. 68.12 ± 10.15, respectively, P 0.05). Conclusion: It seems that alginate can prevent premature acrosome reaction and protect sperm DNA from denaturation during the rapid freezing process.
Keywords :
Alginate , Encapsulation , Rapid Freezing , Sperm DNA
