Title of article :
Trehalose increase proliferation and decrease apoptosis in hair cells induced by Hydrogen peroxides
Author/Authors :
Abbaszadeh, Hojjat Allah Hearing disorders research center - Loghman Hakim Hospital - Shahid Beheshti University of Medical Sciences - Tehran, Iran , hirazi Tehrani, Atefeh Department of Biology and Anatomy - Shahid Beheshti University of Medical Sciences - Tehran, Iran , Niknazar, Somayeh Hearing disorders research center - Loghman Hakim Hospital - Shahid Beheshti University of Medical Sciences - Tehran, Iran , Khoshsirat, Shahrokh Hearing disorders research center - Loghman Hakim Hospital - Shahid Beheshti University of Medical Sciences - Tehran, Iran
Abstract :
BACKGROUND: Hydrogen peroxide (H2O2) a key reactive oxygen species, which is made through redox procedure, can induce oxidative damage to several biological macromolecules and play critical role in neurodegenerative diseases.
Aim: The current study examined the neuroprotective effect of Trehalose on hair cells death induced by H2O2.
Material and method: Hair cells were co-treated by Trehalose and H2O2. The effects of Trehalose on BAX/BCL2 expression ratio and cell viability were assessed by Real time PCR and MTT assay, respectively.
Result: The results of Real time PCR and MTT assay indicated that H2O2 induce cell death, and Trehalose have neuroprotective effect and decreases cell death.
Conclusion: Our data showed that Trehalose has the protective effect on hair cells death induced by H202
Keywords :
Hair cells , H2O2 , Trehalose , BAX/BCL2 expression
Journal title :
Otorhinolaryngology and Facial Plastic Surgery
