The Effect of four Weeks of Continuous Aerobic Training and Starvation on the Expression of Gene Pink1 and Bnibp3 in liver Tissue, liver Enzymes and lipid profile in Wistar Fatty Model Rats

Background and objectives: Regular exercise and calorie intake promote mitochondrial function by promoting healthy mitochondrial regeneration. The present study aimed to investigate the effects of four weeks of continuous aerobic training and starvation on the gene expression of Pink1 and Bnibp3 in liver Tissue, liver Enzymes, and lipid profile in Wistar Fatty Model Rats. Material and Methods: Thirty 18-week-old Fatty Model male Wistar rats with an average body weight of 348±25.53 were selected and randomly divided into six groups of 5, control (n=5) and experimental (n=25), including starvation, starvation, and three days of exercise, starvation, and five days of exercise, three days of exercise, five days of exercise groups. Calorimetric methods measured biochemical factors, including lipid profile. Real-time RT-PCR was used to quantify the mRNA expression of genes. Results: There was a significant decrease in triglyceride (P=0.00), cholesterol (P=0.001), liver enzymes ALT (P=0.001), and AST (P=0.00) in all groups compared to the controls. Furthermore, there was a significant increase in Pink1 gene expression in the starvation group (P=0.00) and starvation groups of 3 and 5 days of training (P=0.00, p=0.00) and in gene expression of Bnibp3 in the starvation group (P=0.00) and starvation groups of 5 days of training (P=0.00) compared to the control group. Conclusion: Four weeks of continuous aerobic training and starvation combined and alone significantly reduced the status of blood lipids and liver enzymes in fatty model rats. The starvation group and starvation groups, along with exercise, can increase the activity of removing damaged mitochondria by elevating the expression of Pink1 and Bnibp3 genes.