Title of article :
Comparison between the rhizosphere microbiome of transgenic sugar beet resistant to rhizomania and non-transgenic parent, Revealed by Illumina Miseq
Author/Authors :
Rouzi Dastenaei, Mina National Institute of Genetic Engineering and Biotechnology, shahrak-e pajoohesh, km15, Tehran-Karaj Highway , Rajaei, Saeideh National Institute of Genetic Engineering and Biotechnology, shahrak-e pajoohesh, km15, Tehran-Karaj Highway , Malboobi, Mohammad Ali National Institute of Genetic Engineering and Biotechnology, shahrak-e pajoohesh, km15, Tehran-Karaj Highway , Ebrahimi, Esmaeil The University of Adelaide - La Trobe University, The University of Melbourne
Abstract :
Due to the development and increasing cultivation of genetically engineered
plants as an integral part of modern agriculture, the biosafety of soil
microorganisms, essential elements of soil fertility, quality, and stability, has been
discussed. To reveal the effect of transgenic plants on soil bio-diversity, it is
necessary to compare the microflora of transgenic and parental plants. In this study,
second-generation GM sugar beet seeds rendered resistant to the propagation of
Necrotic yellow vein virus of beets (BNYVV) via gene silencing in a field trial were
compared to their parental plant by analyzing 16S rDNA metagenomes with the
use of the Illumina MiSeq platform. Analysis of the alpha and beta diversity found
some influence on bacterial communication of rhizosphere between non-transgenic
and transgenic (including 211S3, 219S3, 228S3, and 231S6 cultivars) sugar beets.
Based on the results of the research on alpha diversity, the transgenic 211S3 and
219 S3 cultivars showed a lower average than the control sample in the Cho,
phylogenetic diversity, Cho1 bias correction, and the number of OTUs indexes;
The transgenic 231S6 cultivar showed a significantly higher mean than the control
sample in the Simpsons index. Also, in the study of beta diversity based on the
Bray-Curtis distance algorithm, all 211S3, 219S3, 228S3, and 231S6 cultivars and
control samples were positioned in one group. According to the Unweighted
UniFrac distance algorithm, 219S3 and 231S6 cultivars were put together in one
group, and 211S3 and 228S3 cultivars were classified into one group. A
comparison of the bacterial genera showed a noteworthy reduction in relative
abundance. While a few genera showed a significant decrease in terms of overall
abundance, other genera that stabilize molecular nitrogen and motivate plant
growth, such as Agrobacterium, Devosia, Mesorhizobium, Burkholderia, and
Bradyrhizobium, showed a significant decrease compared to the control cultivar in
all transgenic beets.
Keywords :
Beet necrotic yellow vein virus , Bacterial community , Genetically modified sugar beet , Illumina MiSeq , Rhizosphere Soil , 16S ribosomal RNA gene
Journal title :
Advanced Research in Microbial Metabolite and Technology
