Synergistic anticancer effect of silver nanoparticles and Measles virus vaccine strain against hormone-dependent and -independent breast cancer cells

Objective(s): This study aimed to prepare a stable colloidal silver nanoparticles (AgNPs) and assess its novelty combination therapy, which comprises AgNPs and lives attenuated Measles virus (MV) vaccine, to target breast cancer cells. The safety of the proposed therapy in normal breast epithelial cell lines (HBL-100) was evaluated. Methods: Silver nanoparticles prepared by chemical reduction, The stability, size, and concentration of the colloidal component have been demonstrated by examining the ultraviolet-visible (UV-Vis) spectroscopy at various times using Zeta potential examination, atomic force microscopy (AFM), and atomic spectroscopy. MV was propagated using the VERO-hSLAM cell line. Cytotoxicity assay was evaluated on human breast cancer cell lines. The safety of the proposed therapy in normal Human breast Luminal epithelial cells was assessed to compare the effect against cancer cell lines. Results: The formation of nanoparticles is confirmed by the appearance of a perfect surface plasmon resonance (SPR) band at 424 nm. The stability was proved via a slight change in the absorption intensity from 224 nm to 226 nm (immediately and after a month), respectively, and the value of the charge was -41.13 mV. NPs were spherical in shape and had an average diameter of 40.87 nm. The concentration was 13 μg/ml. The Chou–Talalay analysis revealed synergism between the Measles virus and silver nanoparticles in all tested cancer cell lines and there were highly significant differences (p-value 0.001) among them Conclusions: The novel combination of AgNPs and MV showed effective antitumor activity against breast cancer cells with high safety in normal human breast cells.