Simultaneous spectrofluorometric analysis of caffeic and ellagic acids through inclusion complex formation with γ-Cyclodextrin

The extraordinary sensitivity of uorescence spectroscopy has brought it to chemists' attention. Spectra recording at several interval times while the reaction is preceded, or at dierent complexing factor concentrations, is one case of policies by which data (second-order) can be created using the spectro uorometric technique. In the current case, a mixed uorescence spectrum of two Caffeic Acid (CA) and Ellagic Acid (EA) target analytes was documented as a -cyclodextrin ( -CD) function (as an inclusion complexing factor) to produce data (second-order). At this point, Bilinear Least Squares/Residual Bilinearization (BLLS/RBL), as a second-order calibration technique, due to its benets of accuracy, rapidity, simplicity, suitable concentration, and spectral resolution estimation even in the presence of the unknown interference (second-order advantage) was exploited for trilinear data deconvolution to obtain uorescence spectra and concentration proles of the EA and CA as a function of -CD concentrations. A set of calibrations comprising 10 reference samples was employed to build the BLLS/RBL procedure. By a test set including 6 samples, the predictive ability of the technique was validated. The suggested model was effectively exploited to quantify CA and EA content in four fruit juice samples.