Author/Authors :
Rabiee, Negar Department of Cell and Molecular Biology - Faculty of Chemistry - University of Kashan - Kashan - Isfahan, Iran , Nakhaei Sistani, Roohollah Department of Cell and Molecular Biology - Faculty of Chemistry - University of Kashan - Kashan - Isfahan, Iran , Ahadi, Ali Mohammad Department of Genetics - Faculty of Science - Shahrekord University - Shahrekord, Iran
Abstract :
Obesity is a multifactorial disorder that has increased dramatically in recent
years in developing countries. The disproportion of energy uptake and
expenditure, which is the result of several factors such as diet, behavior,
environment, as well as metabolic and genetic factors, could eventually lead to
this disease. Mutations in genes that regulate appetite and metabolism could
affect various aspects of obesity. Adiponectin protein, the product of the
ADIPOQ gene, is an essential adipokine for controlling energy homeostasis
and fat storage and is secreted in white adipose tissues. It is an insulin-sensitive
hormone whose blood concentration declines in obesity. In this study, we
examined the association of rs2241766 +45 T>G polymorphism of the
ADIPOQ gene with obesity and blood levels of High-Density Lipoprotein
(HDL), Low-Density Lipoprotein (LDL), Triglyceride (TG), and total
cholesterol in the population of Borujen City. In this case-control study, blood
samples were collected from people referred to Tamin Ejtemaee Laboratory of
Borujen who suffered from obesity problems. Triglyceride, total cholesterol,
LDL, and HDL levels were analyzed in the patient's blood using the alpha6
auto-analyzer. In the next step, total DNA was extracted from the blood
samples and subjected to Restriction Fragment Length Polymorphism (RFLP)
methods to investigate the rs2241766 polymorphism of the ADIPOQ gene.
According to the results of this study, there is no significant correlation
between any alleles and genotypes of this polymorphism with obesity (Chisquare
p-value was 0.82 and 0.85 respectively for alleles and genotypes, oddsratio
1.155, 95% CI= 0.32-4.14). There is no significant correlation between
the blood lipid characteristics of the patients and the genotypes of the studied
polymorphism. According to our results, it seems that the alleles and genotypes
of the ADIPOQ gene rs2241766 do not affect the etiology of obesity in our
samples
Keywords :
ADIPOQ gene , Cholesterol level , Obesity , Polymorphism
