Phytochemical Analysis, GCMS Identification, and Estimation of Antioxidant Activity of Iraqi Vitex negundo L.

Vitex negundo Linn, known as chaste tree, belongs to Verbenaceae family. It is a woody medicinal tree; an aromatic and flowering-ornamental plant has different ethnobotanical and pharmacological uses. The aims of this study included phytochemical analysis, detection of two flavonoids by TLC, GC/MS analysis of hexane extract, and the antioxidant estimation in leaves of Iraqi Vitex negundo activity. The results showed the important phytochemicals in hydroalcoholic and hexane extracts. In addition, the main constituents obtained by GC MS were Sabinene 3.23% and Pinene 0.87% as monoterpenes, alpha-Farnesene 0.5%, Pregnan-3,11-diol-20-one 1.55%, and alpha- Tocopherol 1.55%. Those Iraqi Vitex negundo L. were considered as an important source for chemical constituents with a significant antioxidant activity at low concentration with IC50 26.7, as compared with ascorbic acid.