Peptide Nucleic Acid for Rapid Gap-selective Hydrolysis of DNA by Ce(IV)/EDTA Complex

When gap-structures are formed in substrate DNA by using peptide nucleic acid (PNA), these sites are rapidly and selectively hydrolyzed by Ce(IV)/EDTA complex. Scission efficiency at the gap-site is 6-fold higher than that obtained by use of the corresponding DNA additives. Site-selectivity is also higher. The DNA/PNA duplexes must take anti-parallel direction here.