Author/Authors :
Shokoohi ، Gholamreza Department of Medical Parasitology and Mycology - School of Medicine - Jahrom University of Medical Sciences , Badali ، Hamid Invasive Fungi Research Center, Faculty of Medicine - Mazandaran university of medical sciences , Ahmadi ، Bahram Department of Medical Laboratory Sciences - Faculty of Paramedical - Bushehr University of Medical Sciences , Satoh ، Kazuo General Medical Education and Research Center - Teikyo University , Nouripour-Sisakht ، Sadegh Cellular and Molecular Research Center - Yasuj University of Medical Sciences , Nikaeen ، Mahnaz Department of Environmental Health Engineering - School of Public Health - Isfahan University of Medical Sciences , Geramishoar ، Mohsen Department of Medical Mycology and Parasitology - School of Public Health - Tehran University of Medical Sciences , Jalalizand ، Nilufar National Institute of Health Research Isfahan Health Research Station - Tehran University of Medical Sciences , Kianipour ، Sahar Department of Parasitology and Mycology - School of Medicine - Isfahan University of Medical Sciences , Mirhendi ، Hossein Department of Medical Parasitology Mycology - School of Medicine - Isfahan University of Medical Sciences , Makimura ، Koichi School of Medicine - Teikyo University
Abstract :
Background and Purpose: The frequency and genetic diversity of black fungi in environmental and clinical settings have not been fully studied in Iran. This study aimed to identify and evaluate intra- and inter-species DNA sequence variation and also understand the phylogenetic relationships of melanized fungi and relatives isolated from different geographical regions of Iran. Materials and Methods: In total, 111 clinical and environmental strains of dematiaceous fungi were isolated, and their internal transcribed spacer ribosomal DNA (rDNA) regions were sequenced and analyzed. Results: An inter-species nucleotide sequence diversity rate of 1 to 464 nucleotides was observed between the species. Intra-species differences were found in the strains of Alternaria alternata, Cladosporium cladosporioides, Alternaria tenuissima, Curvularia spicifera, Aureobasidium pullulans, Curvularia hawaiiensis, Neoscytalidium dimidiatum, Alternaria terricola, Alternaria chlamydospora, Didymella glomerata, and Drechslera dematioidea by 0–59, 0–22, 0–4, 0–4, 0–3, 0–2, 0–2, 0–2, 0–2, 0–1, and 0–1 nucleotide, respectively. Conclusion: The internal transcribed spacer rDNA is useful for the discrimination of several taxa of dematiaceous fungi. However, a better understanding of the taxonomy of species of Alternaria requires a larger rDNA region or a library of other gene sequences.
Keywords :
Dematiaceous fungi , Iran , ITS rDNA region , Molecular identification
